Bovine Serum Albumin as A Superior “FP-Tag” for High-Throughput Glycosyltransferase Assay

24 August 2020, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


Here, we report the development of a high-throughput “FP-Tag” OGT assay with bovine serum albumin as a low-cost and superior “FP-Tag”. With this assay, 2-methyleurotinone was identified as a low M-range OGT inhibitor. This type of assay with BSA as “FP-Tag” would find more applications with other glycosyltransferases.


High Throughput Method
Glycosyltransferase Assay
Enzyme Inhibitors
O-GlcNAc transferase

Supplementary materials

BSA as FP Tag SI


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