Analytical Chemistry

Simultaneous Characterization of Lipid sn-Positional and Double-Bond Positional Isomers using On-Demand Electrochemical Mass Spectrometry

Authors

Abstract

We present an on-demand electrochemical mass spectrometry workflow for lipid structural characterization with the capability of identifying double-bond and sn-positional isomers. Two voltage-controlled electrochemical reactions, interfacial electro-epoxidation and anodic corrosion of a cobalt electrode, are cascaded in this strategy. The epoxidized products and Co-adducted ions of lipids are fragmented in tandem MS to generate structure characteristic fragments that indicate double-bond and sn-positions. The unique feature of this workflow lies in the sequential localization of double bonds, elucidation of sn-positions, and collection of native lipid information in a single system and simply by tuning ESI voltages. This feature, as well as low sample consumption, no need for extra apparatus, and quantitative analysis, should allow wide applications in the lipidomic field.

Content

Thumbnail image of Manu_sn-lipid_submitted to ChemRxiv.pdf

Supplementary material

Thumbnail image of SI_sn-lipid_submitted to ChemRxiv.pdf
SI sn-lipid submitted to ChemRxiv