Analytical Chemistry

Coupling 193 nm Ultraviolet Photodissociation and Ion Mobility for Sequence Characterization of Conformationally-Selected Peptides


Ultraviolet photodissociation (UVPD) has emerged as a useful technique for characterizing peptide, protein, and protein complex primary and secondary structure. We present here the modification of a commercial ion mobility (IM) mass spectrometer to incorporate 193 nm UVPD following IM separation, enabling IM-UVPD to fragment species selected by both their m/z (via quadrupole selection) and conformation (mobility-selection).


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Supplementary material

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