CNC-Milled Superhydrophobic Macroporous Monoliths for 3D Cell Culture

02 June 2020, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

High-strength macroporous monoliths can be obtained by simply mixing boehmite nanofiber aqueous acetate dispersions with methyltrimethoxysilane. On the boehmite nanofiber-polymethylsilsesquioxane monoliths, we can fabricate structures smaller than a millimeter in size by computer numerical control (CNC) milling, resulting in a machined surface that is superhydrophobic and biocompatible. Using this strategy, we fabricated a superhydrophobic multiwell plate which holds water droplets to produce 3D cell culture environments for various cell types. We expect these superhydrophobic monoliths to have future applications in 3D tissue construction.

Keywords

macroporous monoliths
composites
CNC milling
superhydrophobic
multicellular tumor spheroids
3D cell cultures

Supplementary materials

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