E. coli Nickel-Iron Hydrogenase 1 Catalyses Non-native Reduction of Flavins: Demonstration for Alkene Hydrogenation by Old Yellow Enzyme

08 May 2020, Version 2
This content is a preprint and has not undergone peer review at the time of posting.


Robust [NiFe] hydrogenase 1 (Hyd1) from Escherichia coli is shown to have non-native, H2-dependent activity for FMN and FAD reduction, and to function as a promising recycling system for FMNH2 supply to flavoenzymes for chemical synthesis, giving a total turnover number over 10 thousand when coupled with an Old Yellow Enzyme ene reductase.


cofactor recycling system
hydrogenation catalyst
Asymmetric Reduction

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