Endowing Nanozymes High Biocatalytic Selectivity by Substrates Channeling and Screening

12 May 2020, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


As an alternative to surmount the weakness of natural enzymes, nanozymes have recently attracted extensive attention from early disease diagnosis to tumor therapy. However, due to the lack of enzyme-like molecular recognition units, the poor substrate selectivity of nanozyme-catalyzed reaction significantly restricts their widespread applications. By biomimicking the ingenuity of metabolism process in living, herein we report a multiple-nanozymes system capable of improving the substrate selectivity via cascade reactions in a confined space. As a showcase, the nitrogen-doped carbon nanocages (NCNC) and Prussian blue nanoparticles (PB NPs) were successfully coupled in a microfluidic device to highly selective oxidation and detection of ascorbic acid (AA, an important electron donor and catalyst for alleviation of oxidative stress) against other interferences. It was revealed that the products of the prior nanozymatic reaction were effectively screened as the reactants of the next one, thus stepwise improving the overall selectivity of the all-nanozyme system. This work may open a new door to improve the selectivity of all-nanozyme-system for prospect applications.


cascade reaction
Confined Space Effect
N-doped carbon
Prussian Blue Nanoparticles
ascorbic acid
microfluidic chip
biomimetic principle

Supplementary materials



Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.