H2 as Fuel for Flavin- and H2O2-Dependent Biocatalytic Reactions

06 May 2020, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


Herein, we report a novel H2-driven regeneration system for reduced flavin cofactors. Using an O2-tolerant hydrogenase, we achieved NAD(P)H-independent, highly selective, ene-reductase-catalyzed C=C-double bond reductions, monooxygenase-catalyzed epoxidations and peroxygenase-catalyzed hydroxylations. The hydrogenase performed up to 46000 catalytic cycles and product titers of up to 22 mM have been accomplished. Overall, this system bears the promise to power flavin-dependent biocatalytic reactions with H2 in a highly atom efficient manner.


Flavin regeneration
enoate reductases
green chemistry


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