Advantageous Antibody Microarray Fabrication Through DNA-Directed Immobilization: A Step Toward Use of Extracellular Vesicles in Diagnostics

04 May 2020, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

An optimized general protocol for DNA-protein ligation is provided and the conjugates are used to convert DNA arrays into antibody microarrays. Arrays obtained through DDI were used to capture and characterize extracellular vesicles (EVs), an emerging class of biomarkers. The proposed platform was tested against commercially available antibody microarrays, showing good performance combined with ease of fabrication.

Keywords

Microarray
DNA-Directed Immobilization
antibody array
DNA
Extracellular Vesicles
Diagnostics

Supplementary materials

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Advantageous antibody microarray fabrication through DNA-directed immobilization a step toward use of extracellular vesicles in diagnostics - Supplementary Information
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