Edward Pallister University of Manchester
High resolution glycan analysis has become an important part of biopharmaceutical API production and quality control. Liquid chromatography (LC) is now a well-established technique in this field but the resolution of similar isomeric glycan structures is still a challenge. Here we show that the addition of ion mobility spectroscopy (IMS) in a hyphenated LC-IMS-MS setting allows for the high resolution of N-glycan isomers during positive ion analysis. We have identified unique features in the IM chromatograms to help differentiate a range of isomeric N-glycans for both RFMS labelled glycans and glycopeptides.
This is an original version of the manuscript
download asset Edward G Pallister Ion Mobility of Glycans chemrxiv.pdf 0.76 MB [opens in a new tab] cloud_download
pdf : 0.76 MB
download asset Edward G Pallister Ion Mobility of Glycans SI chemrxiv.pdf 1 MB [opens in a new tab] cloud_download
pdf : 1 MB
Edward G Pallister Ion Mobility of Glycans SI chemrxiv