Ion-Mobility Mass Spectrometry of Glycans and Glycopeptides Using Both Intact and Glycan Sequencing Approaches In Positive Ion Mode Facilitates Glycan Isomer and Glycan Motif Identification

20 April 2020, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


High resolution glycan analysis has become an important part of biopharmaceutical API production and quality control. Liquid chromatography (LC) is now a well-established technique in this field but the resolution of similar isomeric glycan structures is still a challenge. Here we show that the addition of ion mobility spectroscopy (IMS) in a hyphenated LC-IMS-MS setting allows for the high resolution of N-glycan isomers during positive ion analysis. We have identified unique features in the IM chromatograms to help differentiate a range of isomeric N-glycans for both RFMS labelled glycans and glycopeptides.


ion mobility spectroscopy
glycan sequencing
HILIC separation
Mass Spectrometric Analysis
database resource

Supplementary materials

Edward G Pallister Ion Mobility of Glycans SI chemrxiv

Supplementary weblinks


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