Biological and Medicinal Chemistry

DNA Translocation Through Vertically Stacked 2D Layers of Graphene & Hexagonal Boron Nitride Heterostructure Nanopore

Ramkumar Balasubramanian Indian Institute of Science, Bangalore


Cost effective, fast and reliable DNA sequencing can be enabled by advances in nanopore based methods, such as the use of atomically thin graphene membranes. However, strong interaction of DNA bases with graphene leads to undesirable effects such as sticking of DNA strands to the membrane surface. While surface functionalization is one way to counter this problem, here we present another solution based on a heterostructure nanopore system, consisting of a monolayer of graphene and hexagonal Boron Nitride (hBN) each. Molecular dynamics studies of DNA translocation through this heterostructure nanopore revealed a surprising and crucial influence of heterostructure layer order in controlling the base specific signal variability. Specifically, the heterostructure with graphene on top of hBN had nearly 3-10x lower signal variability than the one with hBN on top of graphene. Simulations point to the role of differential underside sticking of DNA bases as a possible reason for the observed influence of layer order. Our studies can guide the development of experimental systems to study and exploit DNA translocation through two-dimensional heterostructure nanopores for single molecule sequencing and sensing applications.


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Supplementary material

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Supporting Info
Thumbnail image of Movie_S1 GrhBN_AT.mp4
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Movie S1 GrhBN AT
Thumbnail image of Movie_S2 GrhBN_CG.mp4
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Movie S2 GrhBN CG
Thumbnail image of Movie_S3 hBNGr_AT.mp4
download asset Movie_S3 hBNGr_AT.mp4 1 MB [opens in a new tab]
Movie S3 hBNGr AT
Thumbnail image of Movie_S4 hBNGr_CG.mp4
download asset Movie_S4 hBNGr_CG.mp4 2 MB [opens in a new tab]
Movie S4 hBNGr CG