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Updating and Extending an UV/Vis Spectroscopy-Based Assay for Monitoring of Transformations Between Nucleosides and Nucleobases

30 March 2020, Version 1
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

In this report we update our recently described method for spectral unmixing-based reaction monitoring of nucleobase cleavage reactions. We present an extended suite of 38 established substrates, recommendations regarding solutions to common problems, further applications and adaptations of the method that necessitate deviation from the previously reported protocol.

Keywords

spectral unmixing
UV/Vis spectroscopy
reaction monitoring
nucleosides
nucleotides
nucleobase
nucleoside phosphorylases

Supplementary materials

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Kaspar 2020 SI Spectral Unmixing
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Now Published

Spectral Unmixing‐Based Reaction Monitoring of Transformations between Nucleosides and Nucleobases [opens in a new tab]
Felix Kaspar, Robert T. Giessmann, Sarah Westarp, Katja F. Hellendahl, Niels Krausch, Isabel Thiele, Miriam C. Walczak, Peter Neubauer, Anke Wagner journal article
ChemBioChem , Volume 21, Issue 18
Online publication date: Jun 18, 2020

Version History

Mar 30, 2020 Version 1

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The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv.12044424.v1
D O I: 10.26434/chemrxiv.12044424.v1 [opens in a new tab]

Funding

Deutsche Forschungsgemeinschaft
390540038
EXC 2008:  UniSysCat
https://app.dimensions.ai/details/grant/grant.8454815 [opens in a new tab]
Deutsche Forschungsgemeinschaft
392246628
Chemo-enzymatic synthesis of selenium-modified nucleosides, nucleotides and nucleic acids
https://app.dimensions.ai/details/grant/grant.7518032 [opens in a new tab]

Author’s competing interest statement

A.W. is the CEO, P.N. is a board member, and F.K. is a scientific researcher in the biotech company BioNukleo GmbH. The authors declare no competing financial interest.

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