Rational Enzyme Design Without Structural Knowledge: A Sequence-Based Approach for Effi-Cient Generation of Glycosylation Catalysts

We present an enzyme engineering approach based solely on amino-acids sequence to convert glycoside hydrolases into transglycosylases. We demonstrate its effectiveness on enzymes form five different glycoside hydrolase families, synthesizing various oligosaccharides containing different α-/β-pyranosides or furanosides in one-step with high yields.