Small Molecule-Inducible RNA-Targeting Systems for Temporal Control of RNA Regulation in Vivo

22 January 2020, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


RNA regulation is critical for gene expression control, but tools to temporally manipulate RNA regulatory mechanisms are lacking. Here, we present small molecule-inducible RNA-targeting effectors based on our previously developed CRISPR/Cas-inspired RNA targeting system (CIRTS), which can trigger RNA editing, degradation, or translation on target transcripts. We go on to show the inducible CIRTS editor can be deployed for RNA base editing in vivo, providing a new set of tools to probe RNA regulatory dynamics and control gene expression.


RNA regulation
gene editing


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