Biological and Medicinal Chemistry

Taurine is Covalently Incorporated into Alpha Tubulin

Dan Sackett Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH

Abstract

We use various methods of high resolution mass spectrometry to demonstrate that taurine is covalently attached to the carboxyl terminus of alpha Tubulin from avian erythrocytes. The alpha Tubulin terminal residue, a tyrosine, must already be removed before covalent addition of taurine, via a known cycle of de-tyrosination and re-tyrosination. Taurination appears to stop this cycle. Taurine is the most abundant free amino acid in the body but this is the first time that it has been shown to be covalently incorporated into any protein.

Version notes

no version notes

Content

Thumbnail image of Taurine Tubulin 2019_12_26.pdf
download asset Taurine Tubulin 2019_12_26.pdf 3 MB [opens in a new tab]

Supplementary material

Thumbnail image of Taurine Tubulin 2019_12_26.docx
download asset Taurine Tubulin 2019_12_26.docx 3 MB [opens in a new tab]
Taurine Tubulin 2019 12 26