Oleg Melnyk CNRS, Université de Lille, INSERM, Institut Pasteur de Lille
Aspartimide formation often complicates the solid phase synthesis of peptides. Much less discussed is the potential occurrence of this side-reaction during the coupling of peptide segments using chemoselective peptide bond forming reactions such as the native chemical ligation and extended methods. Here we describe how to manage this problem using bis(2-sulfenylethyl)amido (SEA)-mediated ligation and SUMO-2/SUMO-3 as protein targets.
download asset Protocol_SUMO.pdf 0.93 MB [opens in a new tab] cloud_download
pdf : 0.93 MB