The Problem of Aspartimide Formation During Protein Chemical Synthesis Using SEA-Mediated Ligation

23 December 2019, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


Aspartimide formation often complicates the solid phase synthesis of peptides. Much less discussed is the potential occurrence of this side-reaction during the coupling of peptide segments using chemoselective peptide bond forming reactions such as the native chemical ligation and extended methods. Here we describe how to manage this problem using bis(2-sulfenylethyl)amido (SEA)-mediated ligation and SUMO-2/SUMO-3 as protein targets.


Small-ubiquitin like modifier
SUMO protease activity
SEA-mediated ligation
aspartimide formation
protein chemical synthesis
peptide ligations


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