Covalent and Non-Covalent Strategies for the Immobilization of Tobacco Etch Virus (TEV) Protease on Superparamagnetic Nanoparticles

Jessica L. Norris; Tulsi Patel; Anvesh K. R. Dasari; Thomas A. Cope; Kwang Hun Lim; Robert Hughes

doi:10.26434/chemrxiv.11365616.v1

Biological and Medicinal Chemistry

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Covalent and Non-Covalent Strategies for the Immobilization of Tobacco Etch Virus (TEV) Protease on Superparamagnetic Nanoparticles

19 December 2019, Version 1

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Abstract

TEV protease fusion proteins were prepared and immobilized on superparamagnetic nanoparticles (SPIONs). Non-covalent (avidin-biotin) and covalent (HALOtag-chloroalkane) strategies were explored for TEV immobilization. HALOtag immobilized TEV protease provided enhance performance in both enzyme retention and reusability. SPION-immobilized proteases could be a convenient and cost-saving tool in the life sciences

Keywords

TEV protease cleavage

superparamagnetic iron oxide nanoparticles

streptavidin affinity strategy

halo-tag

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Covalent and non-covalent strategies for the immobilization of Tobacco Etch Virus protease (TEVp) on superparamagnetic nanoparticles

Jessica L. Norris, Tulsi Patel, Anvesh K.R. Dasari, Thomas A. Cope, Kwang Hun Lim, Robert M. Hughes journal article

Journal of Biotechnology , Volume 322

Print publication date: Oct, 2020

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Dec 19, 2019 Version 1

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The content is available under CC BY NC ND 4.0

DOI

10.26434/chemrxiv.11365616.v1

Author’s competing interest statement

No conflict of interest

Covalent and Non-Covalent Strategies for the Immobilization of Tobacco Etch Virus (TEV) Protease on Superparamagnetic Nanoparticles

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Author’s competing interest statement

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