Third-Generation Method for High Throughput Quantification of Trace Palladium by Color or Fluorescence

25 October 2019, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


Chemists frequently encounter problems associated with trace palladium in synthetic samples because palladium is presumably the most frequently used transition metal in synthetic organic chemistry. We previously reported a colorimetric method for trace palladium quantification, the only high throughput method implemented in the pharmaceutical industry. However, slight changes from the published reaction conditions have caused reproducibility problems, with little understanding of underlying molecular mechanisms. In the current study, we took a combinatorial approach to investigate the method and found that strong basicity was a culprit for the lack of reproducibility. We changed the reaction conditions and procedure accordingly, which substantially improved reproducibility. We discovered that the reaction followed Michaelis-Menten kinetics, allowing for predicting reaction rates on the basis of the substrate concentrations. The current method showed good correlation with inductively coupled plasma mass spectroscopy when 14 synthetic samples with unknown amounts of trace palladium were quantified.


fluorometric method
colorimetric method


Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.