Tyrosinase-Mediated Oxidative Coupling of Tyrosine Tags on Peptides and Proteins

09 October 2019, Version 1
This content is a preprint and has not undergone peer review at the time of posting.


We report a strategy for chemical protein modification by using tyrosinase enzymes to oxidize exposed tyrosine residues on protein N or C-termini. We explore the chemical space for coupling partners in this reaction and find combinations that can proceed in near quantitative conversion. This strategy is used to conjugate a dye onto a Trastuzumab antibody fragment and a Protein L fragment and demonstrate that these constructs can be used as immunostaining reagents.


tyrosinase activity
bioconjugation strategy
bioconjugation techniques
trastuzumab fragments
trastuzumab conjugate
oxidative coupling
Oxidative Couplings Catalyzed

Supplementary weblinks


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