Sortase-Modified Cholera Toxoids Show Specific Golgi Localization

29 July 2019, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Cholera toxoid is an established tool for use in cellular tracing in neuroscience and cell biology. We use a sortase-labelling approach to generate site-specifically N-terminally modified variants of both the A2-B5 heterohexamer and B5 pentamer forms of the toxoid. Both forms of the toxoid are endocytosed by GM1-positive mammalian cells, and while the heterohexameric toxoid was principally localized in the ER, the B5 pentamer showed an unexpected localization in the medial/trans Golgi. This study suggests a future role for specifically-labelled cholera toxoids in live-cell imaging beyond their current applications in neuronal tracing and labelling of lipid-rafts in fixed cells.

Keywords

Cholera Toxin
Sortase
Protein Labelling
Cellular Imaging
Golgi body

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