Biological and Medicinal Chemistry

Straightforward Background Depletion Unlocks Transaminase Evolution Through Solid-Phase Screening



Here we report a rapid, reliable, and widely applicable solid-phase screening method for transaminase variants, which was successfully applied to a transaminase from Halomonas elongata (HEWT), evolved through rounds of random mutagenesis towards a series of diverse prochiral ketones. Key element of this work is the effective and long lasting background depletion which enhances discrimination and minimised false positive hits.


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Supplementary material

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