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Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells

29 November 2018, Version 1
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Norbornene derivatives were validated as probes for cysteine sulfenic acid on proteins and in live cells. Trapping sulfenic acids with norbornene probes is highly selective and revealed a different reactivity profile than the traditional dimedone reagent. The norbornene probe also revealed a superior chemoselectivity when compared to a commonly used dimedone probe. Together, these results advance the study of cysteine oxidation in biological systems.

Keywords

cysteine sulfenic acid
cysteine
protein
protein oxidation

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Now Published

Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells [opens in a new tab]
Lisa J. Alcock, Bruno L. Oliveira, Michael J. Deery, Tara L. Pukala, Michael V. Perkins, Gonçalo J. L. Bernardes, Justin M. Chalker journal article
ACS Chemical Biology , Volume 14, Issue 4
Online publication date: Mar 20, 2019

Version History

Nov 29, 2018 Version 1

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The content is available under CC BY 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv.7396958.v1
D O I: 10.26434/chemrxiv.7396958.v1 [opens in a new tab]

Funding

Australian Research Council (DE150101863)
The Royal Society (UF110046 and URF\R\180019)
FCT Portugal (iFCT, IF/00624/2015)
European Research Council (Starting Grant No. 676832)
Marie Sklodowska-Curie ITN Protein Conjugates (No. 67500)
EPSRC
Australian Government Endeavour Postgraduate Scholarship

Author’s competing interest statement

No conflict of interest

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