Raphael LABRUERE Université Paris Saclay
This study describes the synthesis of fluorescent probes as potential substrates for the polyamine transport system (PTS) of Leishmania donovani. A competitive radioassay was used to determine the most efficient probe. We observed that the conjugate spermine-nitrobenzofurazan (Spm-NBD) was able to compete with [3H]-spermidine in L. donovani at a relevant IC50 of 60 µM.
download asset ChemRxiv_Jagu et al.pdf 1 MB [opens in a new tab] cloud_download
pdf : 1 MB