Spermine-NBD as Fluorescent Probe for Studies of Polyamine Transport System in Leishmania Donovani

Raphael LABRUERE; Sébastien Pomel; Elodie Jagu; Jean-Christophe Cintrat; Philippe M. Loiseau; stéphanie Pethe

doi:10.26434/chemrxiv.7207448.v1

Biological and Medicinal Chemistry

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Spermine-NBD as Fluorescent Probe for Studies of Polyamine Transport System in Leishmania Donovani

16 October 2018, Version 1

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Abstract

This study describes the synthesis of fluorescent probes as potential substrates for the polyamine transport system (PTS) of Leishmania donovani. A competitive radioassay was used to determine the most efficient probe. We observed that the conjugate spermine-nitrobenzofurazan (Spm-NBD) was able to compete with [³H]-spermidine in L. donovani at a relevant IC₅₀ of 60 µM.

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Spermine-NBD as fluorescent probe for studies of the polyamine transport system in Leishmania donovani

Elodie Jagu, Sébastien Pomel, Stéphanie Pethe, Jean-Christophe Cintrat, Philippe M. Loiseau, Raphaël Labruère journal article

Bioorganic & Medicinal Chemistry Letters , Volume 29, Issue 14

Print publication date: Jul, 2019

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Oct 16, 2018 Version 1

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The content is available under CC BY NC ND 4.0

DOI

10.26434/chemrxiv.7207448.v1

Funding

This work was supported by Initiative d’Excellence Paris-Saclay (IdEx)

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None

Spermine-NBD as Fluorescent Probe for Studies of Polyamine Transport System in Leishmania Donovani

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Author’s competing interest statement

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