Physical Chemistry

Fluorescence Enhancement of a Microbial Rhodopsin via Electronic Reprogramming

Abstract

The manuscript reports on two mutations of the photo-sensory protein Anabaena Sensory Rhodopsin and how these mutations modify the fluorescence quantum yield with respect to the wild-type protein. Experimental results are presented and explained theoretically on the basis of mixing of the S1 and S2 excited states. This mixing modulated by electrostatic and steric effects, tunes the excited state potential energy surface, and thereby the excited state lifetime and the fluorescence quantum yield.

Version notes

Version submitted to JACS, under review

Content

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Supplementary material

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JACS FLUORESCENCE SI final