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pH-mediated molecular differentiation for fluorimetric quantification of chemotherapeutic drugs in human plasma

preprint
submitted on 04.12.2017, 12:06 and posted on 05.12.2017, 14:33 by Luis A Serrano, Ye Yang, Elisa Salvati, Francesco Stellacci, Silke Krol, Stefan Guldin
At present, drug dosage is based on standardised approaches that disregard pharmakokinetic differences between patients and lead to non-optimal efficacy and unnecessary side effects. In this work, we demonstrate the potential of pH-mediated fluorescence spectroscopy for therapeutic drug monitoring in complex media. We apply this principle to the simultaneous quantification of the chemotherapeutic prodrug Irinotecan and its active metabolite SN-38 from human plasma across the clinically relevant concentration range, i.e. from micromolar to nanomolar at molar ratios of up to 30:1.

Funding

EC H2020 633635 (DIACHEMO)

History

Topic

  • Analytical Chemistry

Email Address of Submitting Author

s.guldin@ucl.ac.uk

Institution

University College London

Country

United Kingdom

ORCID For Submitting Author

0000-0002-4413-5527

Declaration of Conflict of Interest

There are no conflicts to declare.

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