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Visualization of Streams of Small Organic Molecules in Continuous-Flow Electrophoresis

preprint
submitted on 19.12.2019 and posted on 23.12.2019 by Nikita A. Ivanov, Sven Kochmann, Sergey N. Krylov
Continuous-flow electrophoresis (CFE) separates a stream of a multi-component mixture into multiple streams of individual components inside a thin rectangular chamber. CFE will be able to benefit flow chemistry when it is capable of generically detecting streams of small organic molecules. Here we propose a general approach for molecular stream visualization via analyte-caused obstruction of excitation of a fluorescent layer underneath the separation chamber – fluorescent sublayer-based visualization (FSV). We designed and fabricated a CFE device with one side made of quartz and another side made of UV-absorbing visibly-fluorescent, chemically-inert, machinable plastic. This device was demonstrated to support non-aqueous CFE of small organic molecules and quantitative detection of their streams in real-time with a limit of detection below 100 µM. Thus, CFE may satisfy conditions required for its seamless integration with continuous flow organic synthesis in flow chemistry.

Funding

NSERC STPG-P 521331-2018

History

Email Address of Submitting Author

skochman@yorku.ca

Institution

York University, Toronto, Ontario

Country

Canada

ORCID For Submitting Author

0000-0001-7423-4609

Declaration of Conflict of Interest

The authors declare no conflict of interest.

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