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Visible-Light Triggered Templated Ligation on Surface Using Furan-Modified PNAs
Preprints are manuscripts made publicly available before they have been submitted for formal peer review and publication. They might contain new research findings or data. Preprints can be a draft or final version of an author's research but must not have been accepted for publication at the time of submission.
submitted on 18.09.2020 and posted on 21.09.2020by Alex Manicardi, Enrico Cadoni, Annemieke Madder
Oligonucleotide-templated reactions are frequently exploited for target detection in biosensors and for the construction of DNA-based materials and probes in nanotechnology. Translation of the specifically used template chemistry from solution to surfaces, with the final aim of achieving highly selective high-throughput systems, has been difficult to reach and poorly explored. Here, we show the first example of a visible light-triggered templated ligation on a surface, employing furan-modified peptide nucleic acids (PNAs). Tailored photo-oxidation of the pro-reactive furan moiety is ensured by the simultaneous introduction of a weak photosensitizer as well as a nucleophilic moiety in the reacting PNA strand. This allows a localized production of singlet oxygen for furan activation, which is not affected by probe dilution or reducing conditions. White light irradiation in combination with recognition of a short 22mer oligo sequence that functions as a template, allows sensitive detection of nucleic acid targets in a 96 well plate format.