ChemRxiv
These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
1/1
0/0

Validating Differential Volatilome Profiles in Parkinson’s Disease

preprint
submitted on 21.06.2020 and posted on 24.06.2020 by Eleanor Sinclair, Caitlin Walton-Doyle, Depanjan Sarkar, Katherine A. Hollywood, Joy Milne, Sze Hwy Lim, Tilo Kunath, Anouk Rijs, R.M.A. de Bie, Monty Silverdale, Drupad Trivedi, Perdita Barran

Parkinson’s disease (PD) is a progressive neurodegenerative disorder that does not currently have a robust clinical diagnostic test. Non-motor symptoms such as skin disorders have long since been associated with the disease and more recently a characteristic odour emanating from the skin of people with Parkinson’s has been identified. Here, dynamic head space (DHS) thermal desorption (TD) gas chromatography-mass spectrometry (GC-MS is implemented to directly measure the volatile components of sebum on swabs sampled from people with Parkinson’s – both drug naïve and those on PD medications (n= 100) and control subjects (n= 29). Supervised multivariate analyses of data showed 84.4 % correct classification of PD cases using all detected volatile compounds. Variable importance in projection (VIP) scores were generated from these data, which revealed eight features with VIP > 1 and p < 0.05 which all presented a downregulation within the control cohorts. Purified standards based on previously annotated biomarkers eicosane and octadecanal did not match to clinical data, although multiple metabolite features are annotated with these compounds all with high spectral matches indicating the presence of a series of similar structured species. DHS-TD-GC-MS analysis of a range of lipid standards has revealed the presence of common hydrocarbon species rather than differentiated intact compounds which are hypothesised to be breakdown products of lipids. This replication study validates that a differential volatile profile between control and PD cohorts can be measured using an analytical method that measures volatile compounds directly from skin swabs.

Funding

Michael J. Fox - Research grant 12921

BBSRC BB/L015048/1

Parkinson UK K-1504

History

Email Address of Submitting Author

eleanor.sinclair@manchester.ac.uk

Institution

University of Manchester

Country

United Kingdom

ORCID For Submitting Author

0000-0003-0698-1105

Declaration of Conflict of Interest

No conflict of interest

Exports

Logo branding

Exports