These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
3 files

Using FRET to Measure the Time it Takes for a Cell to Destroy a Virus

submitted on 13.11.2019, 15:35 and posted on 22.11.2019, 01:54 by Candace E. Benjamin, Zhuo Chen, Olivia Brohlin, Hamilton Lee, Stefanie Boyd, Duane Winkler, Jeremiah J. Gassensmith

The emergence of viral nanotechnology over the preceding two decades has created a number of intellectually captivating possible translational applications; however, the in vitro fate of the viral nanoparticles in cells remains an open question. Herein, we investigate the stability and lifetime of virus-like particle (VLP) Qβ - a representative and popular VLP for several applications - following cellular uptake. By exploiting the available functional handles on the viral surface, we have orthogonally installed the known FRET pair, FITC and Rhodamine B, to gain insight of the particle’s behavior in vitro. Based on these data, we believe VLPs undergo aggregation in addition to the anticipated proteolysis within a few hours of cellular uptake.


Graduate Research Fellowship Program (GRFP)

Directorate for Education & Human Resources

Find out more...

CAREER: Viral Capsids as Smart Nanocontainers

Directorate for Mathematical & Physical Sciences

Find out more...

Welch Foundation AT-1989-20190330


Email Address of Submitting Author


University of Texas at Dallas



ORCID For Submitting Author


Declaration of Conflict of Interest


Version Notes

version 1 w/o SI