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Using FRET to Measure the Time it Takes for a Cell to Destroy a Virus

preprint
submitted on 13.11.2019 and posted on 22.11.2019 by Candace E. Benjamin, Zhuo Chen, Olivia Brohlin, Hamilton Lee, Stefanie Boyd, Duane Winkler, Jeremiah J. Gassensmith

The emergence of viral nanotechnology over the preceding two decades has created a number of intellectually captivating possible translational applications; however, the in vitro fate of the viral nanoparticles in cells remains an open question. Herein, we investigate the stability and lifetime of virus-like particle (VLP) Qβ - a representative and popular VLP for several applications - following cellular uptake. By exploiting the available functional handles on the viral surface, we have orthogonally installed the known FRET pair, FITC and Rhodamine B, to gain insight of the particle’s behavior in vitro. Based on these data, we believe VLPs undergo aggregation in addition to the anticipated proteolysis within a few hours of cellular uptake.

Funding

Graduate Research Fellowship Program (GRFP)

Directorate for Education & Human Resources

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CAREER: Viral Capsids as Smart Nanocontainers

Directorate for Mathematical & Physical Sciences

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Welch Foundation AT-1989-20190330

History

Email Address of Submitting Author

gassensmith@utdallas.edu

Institution

University of Texas at Dallas

Country

USA

ORCID For Submitting Author

0000-0001-6400-8106

Declaration of Conflict of Interest

none

Version Notes

version 1 w/o SI

Exports