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Using Collision Cross Section Distributions to Assess the Distribution of Collision Cross Section Values

preprint
revised on 16.01.2020 and posted on 20.01.2020 by Aidan France, Lukasz Migas, Eleanor Sinclair, Bruno Bellina, Perdita Barran
In this study we explore the use of collision cross section distributions to allow comparability of IM-MS data for proteins on different instruments. We present measurements on seven standard proteins across three IM-MS configurations, namely an Agilent 6560 IM QToF, a Waters Synapt G2 possessing a TWIMS cell and a modified Synapt G2 possessing an RF confining linear field drift cell. Mobility measurements were taken using both He and N2 as the drift gases. To aid comparability across instruments and best assess the corresponding gas-phase conformational landscapes of the protein ‘standards’ we present the data in the form of averaged collision cross section distributions.

Funding

This research was supported by the BBSRC (awards: BB/L015048/1, BB/K017802/1 and BB/H013636/1 and the BBSRC/EPSRC-funded Manchester Synthetic Biology Research Centre, SYNBIOCHEM (BB/M017702/1)) and the EPSRC Future Biomanufacturing Research Hub EP/S01778X/1, and the Centre for Biocatalytic Manufacture of New Modalities (CBNM) EP/S005226/1. MRC, BBSRC, EPSRC, Waters Corp. Agilent and the Universities of Edinburgh and Manchester are thanked for their support of studentships to AF, LGM, and ES.

History

Email Address of Submitting Author

Perdita.barran@manchester.ac.uk

Institution

The University of Manchester

Country

UK

ORCID For Submitting Author

0000-0002-7720-586X

Declaration of Conflict of Interest

No COI

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