ChemRxiv
These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
1/1
2 files
0/0

Updating and Extending an UV/Vis Spectroscopy-Based Assay for Monitoring of Transformations Between Nucleosides and Nucleobases

preprint
submitted on 28.03.2020 and posted on 30.03.2020 by Felix Kaspar, Robert T. Giessmann, Sarah Westarp, Katja Hellendahl, Niels Krausch, Isabel Thiele, Miriam C. Walczak, Peter Neubauer, Anke Kurreck
In this report we update our recently described method for spectral unmixing-based reaction monitoring of nucleobase cleavage reactions. We present an extended suite of 38 established substrates, recommendations regarding solutions to common problems, further applications and adaptations of the method that necessitate deviation from the previously reported protocol.

Funding

EXC 2008:  UniSysCat

Deutsche Forschungsgemeinschaft

Find out more...

Chemo-enzymatic synthesis of selenium-modified nucleosides, nucleotides and nucleic acids

Deutsche Forschungsgemeinschaft

Find out more...

History

Email Address of Submitting Author

f.kaspar@tu-bs.de

Institution

Technische Universität Berlin, Institute of Biotechnology, Chair of Bioprocess Engineering

Country

Germany

ORCID For Submitting Author

0000-0001-6391-043X

Declaration of Conflict of Interest

A.W. is the CEO, P.N. is a board member, and F.K. is a scientific researcher in the biotech company BioNukleo GmbH. The authors declare no competing financial interest.

Exports