Updating and Extending an UV/Vis Spectroscopy-Based Assay for Monitoring of Transformations Between Nucleosides and Nucleobases

In this report we update our recently described method for spectral unmixing-based reaction monitoring of nucleobase cleavage reactions. We present an extended suite of 38 established substrates, recommendations regarding solutions to common problems, further applications and adaptations of the method that necessitate deviation from the previously reported protocol.