These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
Tyrosinase-Mediated OC of GGY Tagged Proteins - Alan - Francis 2019.pdf (10.4 MB)

Tyrosinase-Mediated Oxidative Coupling of Tyrosine Tags on Peptides and Proteins

submitted on 05.10.2019, 03:01 and posted on 09.10.2019, 20:04 by Alan Marmelstein, Marco Lobba, Casey S. Mogilevsky, Johnathan Maza, Daniel D. Brauer, Matthew Francis
We report a strategy for chemical protein modification by using tyrosinase enzymes to oxidize exposed tyrosine residues on protein N or C-termini. We explore the chemical space for coupling partners in this reaction and find combinations that can proceed in near quantitative conversion. This strategy is used to conjugate a dye onto a Trastuzumab antibody fragment and a Protein L fragment and demonstrate that these constructs can be used as immunostaining reagents.


Email Address of Submitting Author


University of California Berkeley


United States

ORCID For Submitting Author


Declaration of Conflict of Interest

The authors declare no conflict of interest.