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Tyrosinase-Mediated OC of GGY Tagged Proteins - Alan - Francis 2019.pdf (10.4 MB)
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Tyrosinase-Mediated Oxidative Coupling of Tyrosine Tags on Peptides and Proteins

preprint
submitted on 05.10.2019 and posted on 09.10.2019 by Alan Marmelstein, Marco Lobba, Casey S. Mogilevsky, Johnathan Maza, Daniel D. Brauer, Matthew Francis
We report a strategy for chemical protein modification by using tyrosinase enzymes to oxidize exposed tyrosine residues on protein N or C-termini. We explore the chemical space for coupling partners in this reaction and find combinations that can proceed in near quantitative conversion. This strategy is used to conjugate a dye onto a Trastuzumab antibody fragment and a Protein L fragment and demonstrate that these constructs can be used as immunostaining reagents.

History

Email Address of Submitting Author

mbfrancis@berkeley.edu

Institution

University of California Berkeley

Country

United States

ORCID For Submitting Author

0000-0003-2837-2538

Declaration of Conflict of Interest

The authors declare no conflict of interest.

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