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Tuneable Thiol-Ene Photo-Crosslinked Chitosan-Based Hydrogels for Biomedical Applications

preprint
submitted on 07.09.2020 and posted on 07.09.2020 by Sarah michel, Sarah E rogers, Wuge H. Briscoe, M. Carmen Galan

Access to biocompatible hydrogels with tuneable properties is of great interest. Here we report the practical synthesis and characterisation of a series of photo-crosslinked chitosan hydrogels from norbornene-functionalised chitosan (CS-nb) and various thiolated crosslinkers. The resulting materials were characterised by NMR, swelling ratio, rheology, SEM and SANS. The new hydrogels exhibit pH- and salt-dependent swelling whilst the macro- and microscale properties can be modulated by the choice and degree of crosslinker or the polymer concentration. The materials can be molded in situ and loaded with small molecules that are released overtime. Moreover, the incorporation of collagen in the hydrogels drastically improves cell adhesion and excellent cell viabilities were observed with human dermofibroblast cells for up to 6 days, highlighting the potential use of these materials in the biomedical area.

Funding

MCG and SM were supported by the European Research Council (ERC−COG: 648239) and the Bristol Chemical Synthesis Centre for Doctoral Training (Engineering and Physical Science Research Council (EPSRC) EP/L015366/1). This publication is based upon work from COST Action GLYCONanoPROBES (CA18132), supported by COST (European Cooperation in Science and Technology).

History

Email Address of Submitting Author

m.c.galan@bristol.ac.uk

Institution

University of Bristol

Country

United Kingdom

ORCID For Submitting Author

0000-0001-7307-2871

Declaration of Conflict of Interest

No conflict of interest to report

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