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Total Correlation Spectroscopy Across All NMR-Active Nuclei by Mixing at Zero Field

preprint
submitted on 12.06.2020, 07:54 and posted on 23.06.2020, 06:25 by Ivan V. Zhukov, Alexey S Kiryutin, Fabien Ferrage, Gerd Buntkowsky, Alexandra V. Yurkovskaya, Konstantin Ivanov
Multidimensional Nuclear Magnetic Resonance (NMR) is based on the combination of well-established building blocks for polarization transfer. These blocks are used to design correlation experiments through one or a few chemical bonds or through space. Here, we introduce a building block that enables polarization transfer across all NMR-active nuclei in a coupled network of spins: isotropic mixing at Zero and Ultra-Low Field (ZULF). Exploiting mixing under ZULF-NMR conditions, heteronuclear TOtal Correlation SpectroscopY (TOCSY) experiments were developed to highlight coupled spin networks. We demonstrate 1H-13C and 1H-15N correlations in ZULF-TOCSY spectra of labelled amino acids, which allow one to obtain cross-peaks among all hetero-nuclei belonging to the same coupled network, even when the direct interaction between them is negligible. We also demonstrate the interest of ZULF-TOCSY to analyze complex mixtures on the supernatant of ISOGRO, a growth medium of isotope-labelled biomolecules. ZULF-TOCSY enables the quick identification of individual compounds in the mixture by their coupled spin networks. The ZULF-TOCSY method will lead to the development of a new toolbox of experiments to analyze complex mixtures by NMR.

History

Email Address of Submitting Author

ivanov@tomo.nsc.ru

Institution

International Tomography Center SB RAS

Country

Russia

ORCID For Submitting Author

0000-0002-5765-3307

Declaration of Conflict of Interest

no conflict of interest to decl

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in The Journal of Physical Chemistry Letters

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