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zulf_tocsy.pdf (1.91 MB)

Total Correlation Spectroscopy Across All NMR-Active Nuclei by Mixing at Zero Field

submitted on 12.06.2020, 07:54 and posted on 23.06.2020, 06:25 by Ivan V. Zhukov, Alexey S Kiryutin, Fabien Ferrage, Gerd Buntkowsky, Alexandra V. Yurkovskaya, Konstantin Ivanov
Multidimensional Nuclear Magnetic Resonance (NMR) is based on the combination of well-established building blocks for polarization transfer. These blocks are used to design correlation experiments through one or a few chemical bonds or through space. Here, we introduce a building block that enables polarization transfer across all NMR-active nuclei in a coupled network of spins: isotropic mixing at Zero and Ultra-Low Field (ZULF). Exploiting mixing under ZULF-NMR conditions, heteronuclear TOtal Correlation SpectroscopY (TOCSY) experiments were developed to highlight coupled spin networks. We demonstrate 1H-13C and 1H-15N correlations in ZULF-TOCSY spectra of labelled amino acids, which allow one to obtain cross-peaks among all hetero-nuclei belonging to the same coupled network, even when the direct interaction between them is negligible. We also demonstrate the interest of ZULF-TOCSY to analyze complex mixtures on the supernatant of ISOGRO, a growth medium of isotope-labelled biomolecules. ZULF-TOCSY enables the quick identification of individual compounds in the mixture by their coupled spin networks. The ZULF-TOCSY method will lead to the development of a new toolbox of experiments to analyze complex mixtures by NMR.


Email Address of Submitting Author


International Tomography Center SB RAS



ORCID For Submitting Author


Declaration of Conflict of Interest

no conflict of interest to decl


Read the published paper

in The Journal of Physical Chemistry Letters