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Time Resolved Transient Circular Dichroism Spectroscopy Using Synchrotron Natural Polarisation

preprint
revised on 20.09.2019, 15:22 and posted on 25.09.2019, 16:41 by François Auvray, David Dennetière, Alexandre Giuliani, Frédéric Jamme, Frank Wien, Bastien Nay, Séverine Zirah, François Polack, Claude Menneglier, Bruno Lagarde, Jonathan D Hirst, Matthieu Réfrégiers

Ultraviolet (UV) synchrotron radiation circular dichroism (SRCD) spectroscopy has made an important contribution to the determination and understanding of the structure of bio-molecules. In this paper, we report an innovative approach that we term time-resolved SRCD (tr-SRCD), which overcomes the limitations of current broadband UV SRCD setups. This technique allows accessing ultrafast time scales (down to nanoseconds), previously measurable only by other methods, such as infrared (IR), nuclear magnetic resonance (NMR), fluorescence and absorbance spectroscopies and small angle X-ray scattering (SAXS). The tr-SRCD setup takes advantage of the natural polarisation of the synchrotron radiation emitted by a bending magnet to record broadband UV CD faster than any current SRCD setup, improving the acquisition speed from 10 mHz to 130 Hz and the accessible temporal resolution by several orders of magnitude. We illustrate the new approach by following the isomers concentration changes of an azopeptide after a photoisomerisation. This breakthrough in SRCD spectroscopy opens up a wide range of potential applications to the detailed characterisation of biological processes, such as protein folding, protein-ligand binding.

Funding

University of Nottingham

Synchrotron SOLEIL

History

Email Address of Submitting Author

matthieu.refregiers@synchrotron-soleil.fr

Institution

Synchrotron SOLEIL

Country

France

ORCID For Submitting Author

0000-0002-3281-2308

Declaration of Conflict of Interest

No conflict of interest

Version Notes

V1.1 revised submission

Exports