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Thiol-Methylsulfone Based Hydrogels: Enhanced Control on Gelation Kinetics for 3D Cell Encapsulation

preprint
submitted on 22.07.2019 and posted on 22.07.2019 by Julieta Paez, Aleeza Farrukh, Małgorzata K. Włodarczyk-Biegun, Aránzazu del Campo
Hydrogels are useful temporal matrices for cell culture technologies. The successful mixing and encapsulation of cells within the gel requires the selection of efficient and cytocompatible gelation reactions occurring in the minute timescale under physiological conditions. The thiol-methylsulfonyl (MS) chemical reaction is introduced here as a novel chemistry to encapsulate cells in polymeric matrices. Thiol-MS crosslinking does not require a light activation step and can occur within the seconds-to-minutes timescale by adjusting the pH in the physiological range 8.0-6.6. This reaction is cytocompatible and the reaction product is hydrolytically stable in cell culture media up to 4 weeks. Cell encapsulation protocols enabling comfortable handling and yielding homogenous distribution of the embedded cells are described. All these features are relevant for the application of this crosslinking reaction to biomedical scenarios. Finally, this manuscript also compares the performance of thiol-MS hydrogels with the established thiol-maleimide and thiol-vinylsulfone hydrogels. The benefit of thiol-MS crosslinking in terms of control over hydrogelation kinetics is demonstrated.

Funding

European Union’s Horizon 2020 research and innovation programme under the FET PROACTIVE grant agreement no. 731957 (Mechano-Control)

DFG (Project no. 422041745)

History

Email Address of Submitting Author

julieta.paez@leibniz-inm.de

Institution

INM – Leibniz Institute for New Materials

Country

Germany

ORCID For Submitting Author

0000-0001-9510-7254

Declaration of Conflict of Interest

No conflict of interest

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