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A theory of reactant-stationary kinetics for a mechanism of zymogen activation

preprint
revised on 10.08.2018, 14:26 and posted on 10.08.2018, 14:45 by Justin Eilertsen, Wylie Stroberg, Santiago Schnell
A theoretical analysis is performed on the nonlinear ordinary differential equations that govern the dynamics of a reaction mechanism of zymogen activation. The reaction consists of a primary non-observable zymogen activation reaction that it is coupled to an indicator (observable) reaction. The product of the first reaction is the enzyme of the indicator reaction, and both reactions are governed by the Michaelis-Menten reaction mechanism. Using singular perturbation methods, we derive asymptotic solutions that are valid under the quasi-steady-state and reactant-stationary assumptions. In particular, we obtain closed form solutions that are analogous to the Schnell-Mendoza equation for Michaelis-Menten type reactions. These closed-form solutions approximate the evolution of the observable reaction and provide the mathematical link necessary to measure the enzyme activity of the non-observable reaction. Conditions for the validity of the asymptotic solutions are also derived, and we demonstrate that these asymptotic expressions are applicable under reactant-stationary kinetics.

Funding

NIH/NIGMS K12 GM111725; University of Michigan Protein Folding Disease Initiative

History

Email Address of Submitting Author

schnells@umich.edu

Institution

University of Michigan Medical School, Ann Arbor, MI

Country

USA

ORCID For Submitting Author

0000-0002-9477-3914

Declaration of Conflict of Interest

No conflict of interest.

Licence

Exports