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The Steadfast Au@Pt Soldier: Peroxide-Tolerant Nanozyme for Signal Enhancement in Lateral Flow Immunoassay of Peroxidase-Containing Samples

preprint
submitted on 22.09.2020 and posted on 23.09.2020 by Vasily Panferov, Irina V. Safenkova, Anatoly V. Zherdev, Boris B. Dzantiev
The approach to inhibit endogenous peroxidases by elevated concentrations of hydrogen peroxide while maintaining the high peroxidase-mimicking activity of Au@Pt nanozymes was developed. The approach facilitates selective and highly-sensitive detection of peroxidase-mimicking nanozyme nanozymes in the background of endogenous peroxidases. Au@Pt nanozyme was used as the colorimetric and catalytic label in lateral flow immunoassay of an important plant pathogen – potato virus X. The inhibition of endogenous peroxidases in plant extracts and selective detection of Au@Pt nanozyme provides the lowest limit of detection among immunochemical assays of potato virus X (up to 500 times lower compared to the assay with conventional gold nanoparticles).
The proposed approach uses the fundamental principle of enzyme inhibition by the substrate. It is universal and applicable to all matrixes with peroxidase activity.

Funding

Russian Science Foundation (grant no. 16-16-04108)

History

Email Address of Submitting Author

vaspanferov@gmail.com

Institution

A.N. Bach Institute of Biochemistry, Research Center of Biotechnology RAS

Country

Russia

ORCID For Submitting Author

0000-0002-4057-7248

Declaration of Conflict of Interest

No conflict of interest

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