ChemRxiv
These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
Protocol_SUMO.pdf (913.22 kB)
0/0

The Problem of Aspartimide Formation During Protein Chemical Synthesis Using SEA-Mediated Ligation

preprint
submitted on 19.12.2019 and posted on 23.12.2019 by Jennifer Bouchenna, Magalie Sénéchal, Hervé Drobecq, Jérôme Vicogne, Oleg Melnyk
Aspartimide formation often complicates the solid phase synthesis of peptides. Much less discussed is the potential occurrence of this side-reaction during the coupling of peptide segments using chemoselective peptide bond forming reactions such as the native chemical ligation and extended methods. Here we describe how to manage this problem using bis(2-sulfenylethyl)amido (SEA)-mediated ligation and SUMO-2/SUMO-3 as protein targets.

History

Email Address of Submitting Author

oleg.melnyk@ibl.cnrs.fr

Institution

CNRS, Université de Lille, INSERM, Institut Pasteur de Lille

Country

France

ORCID For Submitting Author

0000-0002-3863-5613

Declaration of Conflict of Interest

The authors declare no conflicts of interest

Exports