These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
2 files

The Pattern of Acetylation Defines the Priming Activity of Chitosan Tetramers

submitted on 21.05.2019, 14:52 and posted on 22.05.2019, 15:26 by Sven Basa, Malathi Nampally, Talita Honorato, Subha Narayan Das, Appa Rao Podile, Nour Eddine El Gueddari, Bruno Maria Moerschbacher
The biological activity of chitosans depends on their degree of polymerization (DP) and degree of acetylation (DA). However, information could also be carried by the pattern of acetylation (PA): the sequence of β-1,4-linked glucosamine (deacetylated/D) and N-acetylglucosamine (acetylated/A) units. To address this hypothesis, we prepared partially-acetylated chitosan oligosaccharides from a chitosan polymer (DA=35%, DPw=905) using recombinant chitosan hydrolases with distinct substrate and cleavage specificities. The mixtures were separated into fractions DP4–DP12, which were tested for elicitor and priming activities in rice cells. We confirmed that both activities were influenced by DP, but also observed apparent DA-dependent priming activity, with the ADDD+DADD fraction proving remarkably effective. We then compared all four mono-acetylated tetramers prepared using different chitin deacetylases and observed significant differences in priming activity. This demonstrates for the first time that PA influences the biological activity of chitosans, which can now be recognized as bona fide information-carrying molecules


Email Address of Submitting Author


WWU Münster, Institute of Plant Biology and Biotechnology



ORCID For Submitting Author


Declaration of Conflict of Interest

no conflict of interest