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The Pattern of Acetylation Defines the Priming Activity of Chitosan Tetramers

preprint
submitted on 21.05.2019 and posted on 22.05.2019 by Sven Basa, Malathi Nampally, Talita Honorato, Subha Narayan Das, Appa Rao Podile, Nour Eddine El Gueddari, Bruno Maria Moerschbacher
The biological activity of chitosans depends on their degree of polymerization (DP) and degree of acetylation (DA). However, information could also be carried by the pattern of acetylation (PA): the sequence of β-1,4-linked glucosamine (deacetylated/D) and N-acetylglucosamine (acetylated/A) units. To address this hypothesis, we prepared partially-acetylated chitosan oligosaccharides from a chitosan polymer (DA=35%, DPw=905) using recombinant chitosan hydrolases with distinct substrate and cleavage specificities. The mixtures were separated into fractions DP4–DP12, which were tested for elicitor and priming activities in rice cells. We confirmed that both activities were influenced by DP, but also observed apparent DA-dependent priming activity, with the ADDD+DADD fraction proving remarkably effective. We then compared all four mono-acetylated tetramers prepared using different chitin deacetylases and observed significant differences in priming activity. This demonstrates for the first time that PA influences the biological activity of chitosans, which can now be recognized as bona fide information-carrying molecules

History

Email Address of Submitting Author

sven.basa@uni-muenster.de

Institution

WWU Münster, Institute of Plant Biology and Biotechnology

Country

Germany

ORCID For Submitting Author

0000-0002-9040-314X

Declaration of Conflict of Interest

no conflict of interest

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