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The Metalloid Reductase of Pseudomonas Moravenis Stanleyae Conveys Nanoparticle Mediated Metalloid Tolerance

preprint
submitted on 14.05.2018 and posted on 15.05.2018 by Richard Nemeth, Mackenzie Neubert, Thomas Ni, Christopher J. Ackerson
In the present work we have identified a glutathione reductase like metalloid reductase (GRLMR) responsible for mediating selenite tolerance in Pseudomonas moravenis stanleyae through the enzymatic generation of Se(0) nanoparticles. This enzyme has an unprecedented substrate specificity for selenodiglutathione (Km= 336 μM) over oxidized glutathione (Km=8.22 mM). This enzyme was able to induce selenite tolerance in foreign bacterial cell lines by increasing the IC90 for selenite from 1.9 mM in cell lacking the GRLMR gene to 21.3 mM for cells containing the GRLMR gene. It was later confirmed by STEM and EDS that Se nanoparticles were absent in control cells and present in cells expressing GRLMR. Structural analysis suggests the lack of a sulfur residue in the substrate/product binding pocket may be responsible for this unique substrate specificity.

History

Email Address of Submitting Author

Richard.Nemeth@colostate.edu

Email Address(es) for Other Author(s)

mpneubert652@gmail.com thomas.w.ni@gmail.com docack@gmail.com

Institution

Colorado State University

Country

United States of America

ORCID For Submitting Author

0000-0003-4451-5041

Declaration of Conflict of Interest

No conflict of Interest

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