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The Influence of Structural Gradients in Large Pore Organosilica Materials on the Capabilities for Hosting Cellular Communities

preprint
submitted on 28.01.2020 and posted on 28.01.2020 by Hannah Bronner, Anna-Katharina Holzer, Alexander Finke, Marius Kunkel, Andreas Marx, Marcel Leist, Sebastian Polarz

Cells exist in the so-called extracellular matrix (ECM) in their native state, and numerous future applications require reliable and potent ECM-mimics. A perspective, which goes beyond ECM emulation, is the design of a host-material with features, which are not accessible in the biological portfolio. Such a feature would, for instance be, the creation of a structural or chemical gradient, and to explore how this special property influences the biological processes. First, we wanted to test if macroporous organosilica materials with appropriate surface modification can act as a host for the implementation of human cells like HeLa or LUHMES. It was possible to use a commercially available polymeric foam as a scaffold and coat it with a layer of a thiophenol-containing organosilica layer, followed by biofunctionalization with biotin using click chemistry and the subsequent coupling of streptavidin - fibronectin to it. More importantly, deformation of the scaffold allowed the generation of a permanent structural gradient. In this work, we show that the structural gradient has a tremendous influence on the capability of the described material for the accommodation of living cells. The introduction of a bi-directional gradient enabled the establishment of a cellular community comprising different cell types in spatially distinct regions of the material. An interesting perspective is to study communication between cell types or to create cellular communities, which can never exist in a natural enviornment.

Funding

Dr. K. H. Eberle Foundation

History

Email Address of Submitting Author

sebastian.polarz@uni-konstanz.de

Institution

University of Konstanz

Country

Germany

ORCID For Submitting Author

0000-0003-1651-4906

Declaration of Conflict of Interest

No conflict of interest

Version Notes

Version Jan. 20. 2020

Exports