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The CryoEM Method MicroED as a Powerful Tool for Small Molecule Structure Determination

preprint
submitted on 17.10.2018 and posted on 17.10.2018 by Christopher G. Jones, Michael W. Martynowycz, Johan Hattne, Tyler J. Fulton, Brian M. Stoltz, Jose A. Rodriguez, Hosea Nelson, Tamir Gonen

In the many scientific endeavors that are driven by organic chemistry, unambiguous identification of small molecules is of paramount importance. Over the past 50 years, NMR and other powerful spectroscopic techniques have been developed to address this challenge. While almost all of these techniques rely on inference of connectivity, the unambiguous determination of a small molecule’s structure requires X-ray and/or neutron diffraction studies. In practice, however, x-ray crystallography is rarely applied in routine organic chemistry due to intrinsic limitations of both the analytes and the technique. Here we report the use of the CryoEM method MicroED to provide routine and unambiguous structural determination of small organic molecules. From simple powders, with minimal sample preparation, we could collect high quality MicroED data from nanocrystals (~100x100x100 nm, ~10–15g) resulting in atomic resolution (<1 Å) crystal structures in minutes.

Funding

R35GM128867

History

Email Address of Submitting Author

hosea@chem.ucla.edu

Institution

University of California, Los Angeles

Country

USA

ORCID For Submitting Author

0000-0002-4666-2793

Declaration of Conflict of Interest

No Conflicts

Exports