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Synthesis of Novel Selenocyanates and Evaluation of Their Effect in Cultured Mouse Neurons Submitted to Oxidative Stress

preprint
submitted on 06.05.2020 and posted on 07.05.2020 by Tiago Elias Allievi Frizon, José H. Cararo, Sumbal Saba, Gustavo C. Dal-Pont, Monique Michels, Hugo de C. Braga, Tairine Pimentel, Felipe Dal-Pizzol, Samira Silva Valvassori, Jamal Rafique

Herein we report the synthesis of novel selenocyanates and assessment of their effect on the oxidative challenge elicited by hydrogen peroxide (H2O2) in cultured mouse neurons. First, α-methylene-β-hydroxy esters were prepared as precursors of allylic bromides. A reaction involving the generated bromides and sodium selenocyanate was conducted to produce the desired selenocyanates (3a-f). We next prepared cultures of neurons from 7-day-old-mice (n = 36). H2O2 (10⁻5 M) was added into the culture flasks as an oxidative stress inducer, alone or combined with one of each designed compounds. PhSe)2 was used as positive control. It was carried out assessment of lipid (thiobarbituric acid reactive species, 4-hydroxy-2’-nonenal, 8-isoprostane), DNA (8-hydroxy-2’-deoxyguanosine) and protein (carbonyl) modification parameters. Finally, catalase and superoxide dismutase activities were also evaluated. Among the compounds, 3b, 3d and 3f exhibited the most pronounced pattern of antioxidant activity, similar to (PhSe)2. These novel aromatic selenocyanates could be promising to be tried in most sophisticated in vitro studies or even at preclinical level.

Funding

CNPq Universal (433896/2018-3)

CAPES – Finance Code 001

UFMS

History

Email Address of Submitting Author

jamal.chm@gmail.com

Institution

Federal University of Mato Grosso do Sul

Country

Brazil

ORCID For Submitting Author

0000-0002-2336-040X

Declaration of Conflict of Interest

The authors declare that there is no conflict of interest regarding the publication of this paper

Exports