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Synthesis and Evaluation of α-Amylase and Lipase Inhibitory Activities of Synthetic Coumarin Derivatives

preprint
submitted on 22.02.2019 and posted on 25.02.2019 by shivaprasad shetty Mangalpady, NV Anil, Anil Kumar HS
This research work involved synthesis of nine Coumarin-hydrazone derivatives and study of their enzyme inhibition activity. The purpose of the study was to investigate the α-amylase and lipase inhibition activity of the synthesized coumarin derivatives. The experiment was performed by taking 5, 10, 50,100 μg/mL of each of the compounds and Acarbose and Orlistat was taken as a positive control. The studies showed that compound 5d (120.6 μM), 5e (111.5 μM) and 5h (124.0 μM) was showing better inhibition activity against α-amylase enzyme, in comparison with the standard (IC50=81.70 μM). The lipase enzyme inhibition revealed that the compounds, 5c (142.3 μM), 5d (82.3 μM) and 5i (93.6 μM) showed better inhibition compared to the standard (IC50=236.4±2.83 μM). The enzyme kinetics study also revealed good results and it has been discussed in the results section.

History

Email Address of Submitting Author

nv.anil@manipal.edu

Institution

Manipal Academy of Higher Education

Country

India

ORCID For Submitting Author

0000-0002-8016-8991

Declaration of Conflict of Interest

No conflict of interest

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