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Straightforward Background Depletion Unlocks Transaminase Evolution Through Solid-Phase Screening

preprint
submitted on 21.03.2019 and posted on 25.03.2019 by Matteo Planchestainer, Eimear Hegarty, Christian Heckmann, Louise Gourlay, Francesca Paradisi
Here we report a rapid, reliable, and widely applicable solid-phase screening method for transaminase variants, which was successfully applied to a transaminase from Halomonas elongata (HEWT), evolved through rounds of random mutagenesis towards a series of diverse prochiral ketones. Key element of this work is the effective and long lasting background depletion which enhances discrimination and minimised false positive hits.

Funding

Biotechnology and Biological Sciences Research Council [grant number BB/ P002536/1]

History

Email Address of Submitting Author

francesca.paradisi@nottingham.ac.uk

Institution

University of Nottingham

Country

United Kingdom

ORCID For Submitting Author

0000-0003-1704-0642

Declaration of Conflict of Interest

The authors declare no conflict of interest

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