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Straightforward Background Depletion Unlocks Transaminase Evolution Through Solid-Phase Screening
preprintsubmitted on 21.03.2019, 15:42 and posted on 25.03.2019, 16:13 by Matteo Planchestainer, Eimear Hegarty, Christian Heckmann, Louise Gourlay, Francesca Paradisi
Here we report a rapid, reliable, and widely applicable solid-phase screening method for transaminase variants, which was successfully applied to a transaminase from Halomonas elongata (HEWT), evolved through rounds of random mutagenesis towards a series of diverse prochiral ketones. Key element of this work is the effective and long lasting background depletion which enhances discrimination and minimised false positive hits.