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2018_Majumder-Chopra_live-cell-functionalization_preprint.pdf (12.9 MB)
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Stable and Reversible Functionalization of the Native Phosphate Groups on Live Cells

preprint
submitted on 02.11.2018 and posted on 05.11.2018 by Joydeb Majumder, Gaurav Chopra
The ability to label live cell surfaces has many applications ranging from in vivo monitoring of cell populations to diagnostics and use of cells as drugs. Thus far, most reported strategies to label cell surfaces are not broadly applicable or easy to use for any cell type as it has relied on engineering cells with artificial moieties or conjugations that may affect cellular function. We provide a general solution to this long-standing problem by developing two-sided functionalization of the phosphate moieties that are ubiquitous on all cells. We show one application of our chemical strategy as a general-purpose live-cell membrane imaging reagent with long-time stability. Our strategy is broadly applicable to imaging, sensing, drug delivery, bioengineering, diagnostics and cell therapy.

Funding

Ralph W. and Grace M. Showalter Research Trust, Jim and Diann Robbers Cancer Research Award

History

Email Address of Submitting Author

gchopra@purdue.edu

Institution

Purdue University

Country

United States

ORCID For Submitting Author

0000-0003-0942-7898

Declaration of Conflict of Interest

None

Exports