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Stable Isotope-Assisted Metabolomics for Deciphering Xenobiotic Metabolism in Mammalian Cell Culture

preprint
revised on 26.02.2020 and posted on 26.02.2020 by Mira Flasch, Christoph Bueschl, Lydia Woelflingseder, Heidi E. Schwartz-Zimmermann, Gerhard Adam, Rainer Schuhmacher, Doris Marko, Benedikt Warth
We present a workflow based on stable isotope-assisted metabolomics and the bioinformatics tool MetExtract II for deciphering xenobiotic metabolites produced by human cells. Its potential was demonstrated by the investigation of the metabolism of deoxynivalenol (DON), an abundant food contaminatn, in a liver cracinoma cell line (HeoG2) and a model for colon carcinoma (HT29). Detected known metabolites included DON-3-sulfate, DON-10-sulfonate, and DON-10-glutathione as well as DON-cysteine. Conjugation with amino acids and antibiotics was confirmed for the first time. The approach allows the untargeted elucidation of human xenobiotic products in tissue culture.

History

Email Address of Submitting Author

benedikt.warth@univie.ac.at

Institution

University of Vienna

Country

Austria

ORCID For Submitting Author

0000-0002-6104-0706

Declaration of Conflict of Interest

Authors declare no conflict of interest

Version Notes

Version1_12112019

Exports