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Spontaneous Emergence of Membrane-Forming Protoamphiphiles from a Lipid-Amino Acid Mixture Under Wet-Dry Cycles

preprint
submitted on 19.10.2020, 10:48 and posted on 19.10.2020, 13:04 by Manesh Joshi, Anupam Sawant, Sudha Rajamani

The prelude to the origin of cellular life on Earth would have involved a fundamental step, that of protocell formation. This involves the coming together of two crucial processes; abiotic synthesis of informational and catalytic polymers, and the assembly of membrane compartments. Mutual interactions between these processes would have likely affected the emergence and early stages of protocell evolution. Previous investigations have predominantly focused on cooperative interactions, often neglecting any competitive behavior that might ensue as ‘counterproductive cross-talk’. However, in a realistic scenario, both cooperative and competitive reactions would have occurred simultaneously in a complex prebiotic soup, generating a plethora of chemical species with their own prebiotic implications. In this study, we followed a systematic and unbiased approach to explore this interdependence. We used a lipid-amino acid system to demonstrate the above-mentioned phenomenon wherein we investigated the effect of a membrane-forming amphiphile on peptide synthesis, under prebiotically plausible conditions.


Interestingly, our study shows the formation of a hitherto unobserved reaction product that could have played a significant role during the emergence of life on the early Earth. We do show that peptide synthesis occurs but with a decrease in the yield. This is due to another concurrent and competing reaction, wherein an amino acid covalently interacts with a phospholipid to generate new amphiphilic species called N-acyl amino acids (NAAs) via an ester-amide exchange process. These NAAs are thermostable and, hence, persistent even at high temperatures. Furthermore, this protoamphiphile is also able to self-assemble into vesicles at acidic pH. Au contraire, fatty acids, a widely accepted constituent of prebiotic compartments, have been shown to generate vesicles only at neutral to alkaline pH. Thus, NAAs could have had a selective advantage over fatty acids to form thermostable protocell compartments under acidic geothermal pool-like conditions, a niche that has gained prominence as one of the important geological settings where life could have originated. Our study underlines the importance of an unbiased exploration of the complex interactions between prebiotic processes, which could potentially open new avenues to solving the origin of life conundrum.

Funding

Department of Biotechnology, Govt. of India [BT/PR19201/BRB/10/1532/2016]

DST-FIST grant SR/FST/LSII-043/2016

UGC, Government of India for fellowship support

History

Email Address of Submitting Author

srajamani@iiserpune.ac.in

Institution

IISER Pune

Country

India

ORCID For Submitting Author

https://orcid.org/0000-0002-6030-495X

Declaration of Conflict of Interest

The authors declare no conflict of interest.

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