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Spermine-NBD as Fluorescent Probe for Studies of Polyamine Transport System in Leishmania Donovani

preprint
submitted on 15.10.2018 and posted on 16.10.2018 by Raphael LABRUERE, Sébastien Pomel, Elodie Jagu, Jean-Christophe Cintrat, Philippe M. Loiseau, stéphanie Pethe
This study describes the synthesis of fluorescent probes as potential substrates for the polyamine transport system (PTS) of Leishmania donovani. A competitive radioassay was used to determine the most efficient probe. We observed that the conjugate spermine-nitrobenzofurazan (Spm-NBD) was able to compete with [3H]-spermidine in L. donovani at a relevant IC50 of 60 µM.


Funding

This work was supported by Initiative d’Excellence Paris-Saclay (IdEx)

History

Email Address of Submitting Author

raphael.labruere@u-psud.fr

Institution

Université Paris Saclay

Country

France

ORCID For Submitting Author

0000-0003-3683-2768

Declaration of Conflict of Interest

None

Exports