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Spermine-NBD as Fluorescent Probe for Studies of Polyamine Transport System in Leishmania Donovani
preprintsubmitted on 15.10.2018, 15:10 and posted on 16.10.2018, 13:27 by Raphael LABRUERE, Sébastien Pomel, Elodie Jagu, Jean-Christophe Cintrat, Philippe M. Loiseau, stéphanie Pethe
This study describes the synthesis of fluorescent probes as potential substrates for the polyamine transport system (PTS) of Leishmania donovani. A competitive radioassay was used to determine the most efficient probe. We observed that the conjugate spermine-nitrobenzofurazan (Spm-NBD) was able to compete with [3H]-spermidine in L. donovani at a relevant IC50 of 60 µM.