These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
Enzyvitands: evolvable biosynthetic and colorimetric chalices
preprintrevised on 11.08.2020, 06:42 and posted on 11.08.2020, 07:03 by Thibaud Rossel, Bing Zhang, Raphael Gobat
The literature is constellated with a wide variety of chemosensors against a plethora of analytes. This seminal library is used to inspire chemists to improve them using chemical synthesis. However, their optimization via chemical synthesis is a difficult task which takes time without the guarantee of final success.We show here that combinatorial chemistry,the use of first and second coordination spheres and the displacement of indicators united within a protein cavity offers an easy-to-assemble colorimetric bio-chemical sensor. It consists only of commercial chemicals. This colorimetric sensor is highly modular, cheap and evolvable. Its X-ray structure reveals the composition of its active site. This allows to design it rationally for the recognition of dopamine with the naked eye. Our bio-sensor therefore resembles a biological receptor for the recognition of neurotransmitters. Its immediate high adaptability and ability to be evolved can be useful for the selective detection of a wide variety of analytes going from small molecules to microorganisms. This discovery therefore makes it possible to dream of new biotechnological or new immunotherapeutic applications.