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Enzyvitands_evolvable_biosynthetic_and_colorimetric_chalices-2020-08-11-06-38.pdf (3.35 MB)
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Enzyvitands: evolvable biosynthetic and colorimetric chalices

preprint
revised on 11.08.2020 and posted on 11.08.2020 by Thibaud Rossel, Bing Zhang, Raphael Gobat
The literature is constellated with a wide variety of chemosensors against a plethora of analytes. This seminal library is used to inspire chemists to improve them using chemical synthesis. However, their optimization via chemical synthesis is a difficult task which takes time without the guarantee of final success.We show here that combinatorial chemistry,the use of first and second coordination spheres and the displacement of indicators united within a protein cavity offers an easy-to-assemble colorimetric bio-chemical sensor. It consists only of commercial chemicals. This colorimetric sensor is highly modular, cheap and evolvable. Its X-ray structure reveals the composition of its active site. This allows to design it rationally for the recognition of dopamine with the naked eye. Our bio-sensor therefore resembles a biological receptor for the recognition of neurotransmitters. Its immediate high adaptability and ability to be evolved can be useful for the selective detection of a wide variety of analytes going from small molecules to microorganisms. This discovery therefore makes it possible to dream of new biotechnological or new immunotherapeutic applications.

Funding

no funding

History

Email Address of Submitting Author

thibaud.rossel@emsp.gfbienne.ch

Institution

Gymnase français de Bienne

Country

Switzerland

ORCID For Submitting Author

0000-0003-0254-5445

Declaration of Conflict of Interest

no conflict of interest

Version Notes

Paper version 1

Exports